Figure 2
(a) Schematic of the single-objective light-sheet used to optically scan the biofilm volume to increase the signal-to-background level and enable single-molecule localization deep inside the biofilm. (b) Schematic of FOV positioning when imaging biofilms. A total of 36 FOVs (10 × 40 µm2, inset) were acquired for each sample, in a 3 × 3 × 4 grid. Imaged regions were separated by ~ 100 µm on the XY-plane. Each region on the XY-plane was imaged at four different depths, separated by 4.5 µm vertically, obtaining a 3D map of the AcrB distribution in the region. Created with BioRender.com.
