Figure 3
(a) Axial density profile of AcrB-PAmCherry in the synthetic biofilm. Protein density is measured at various distances from the glass-sample interface (0, 4.5, 9 and 13.5 µm), normalized on the value at 0 µm. Each value is the weighted average over 9 different FOV, laying at the same depth, for three independent biological replicates. Error bars are error of the weighted average. (b) Axial density profile of AcrB-PAmCherry in real biofilms. Data was corrected by taking into account values in (a) as reported in the main text and in Supplementary Materials. Protein density is measured at various distances from the glass-sample interface (0, 4.5, 9 and 13.5 µm), normalized on the value at 0 µm. Each value is the weighted average over 9 different FOV, laying at the same depth, for three independent biological replicates. Error bars are error of the weighted average. Gray bar is protein density in planktonic bacteria, orange bars are protein densities in 24 h grown biofilms and blue bars are protein densities in 48 h grown biofilms. Significance are Student’s t-test p-values calculated with the OriginLab software.
