Figure 1 | Scientific Reports

Figure 1

From: ProteinCoLoc streamlines Bayesian analysis of colocalization in microscopic images

Figure 1

Result of exemplary analysis 1—colocalization between two polyclonal antibodies binding HAP40. (A) Co-staining of HAP40 with the anti-HAP40 antibody sc-69489 (Donkey anti-goat Alexa Fluor 555, red) and anti-Strep antibody (Donkey anti-mouse Alexa-Fluor 468). DNA was stained with DAPI and is depicted in blue. (B) Local correlation plot between the signal from the anti-HAP40 antibody sc-69489 and anti-Strep antibody. (C) Bayes factor plot displaying the prior and posterior distribution for the global \({\Delta }\rho\) between control images (i.e. non-transfected cells, N = 6) and the images from the co-staining (N = 6). (D) Bayes factor range plot displaying the Bayes factor at different thresholds for \({\Delta }\rho\).

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