Figure 4
From: ProteinCoLoc streamlines Bayesian analysis of colocalization in microscopic images
Result of exemplary analysis 2—colocalization between HAP40-Strep and full-length 17QHTT-FLAG HAP40. (a) Co-staining of HAP40 with the anti-HAP40 antibody sc-69489 (Donkey anti-goat Alexa Fluor 555, red) and anti-FLAG antibody (Donkey anti-mouse Alexa-Fluor 468). DNA was stained with DAPI and is depicted in blue. (b) Local correlation plot between the signal from the anti-HAP40 antibody sc-69489 and anti-Strep antibody. (c) Bayes factor plot displaying the prior and posterior distribution for the global \({\Delta }\rho\) between control images (i.e. non-transfected cells, N = 6) and the images from the co-staining (N = 6). (d) Bayes factor range plot displaying the Bayes factor at different thresholds for \({\Delta }\rho\).
