Figure 6 | Scientific Reports

Figure 6

From: MARK4 aggravates cardiac dysfunction in mice with STZ-induced diabetic cardiomyopathy by regulating ACSL4-mediated myocardial lipid metabolism

Figure 6Figure 6

Transcriptomic analysis and sequencing validation of the effects of MARK4 deficiency. (A) Volcano plot of the control group and Hp + PA group (n = 3 samples per group). (B) Volcano plot of the Hp + PA group and Hp + PA + shMARK4 group (n = 3 samples per group). (C) The upregulated genes in the control group and Hp + PA group and downregulated genes in the Hp + PA group and Hp + PA + shMARK4 group are shown as a cross-Venn diagram (n = 3 samples per group). (D) KEGG classification diagram of the differentially expressed genes (n = 3 samples per group). (E) KEGG bubble diagram of the differentially expressed genes (n = 3 samples per group). (F) Heatmap of differentially expressed genes (n = 3 samples per group) (The heatmap analysis software: DESeq2(1.30.1) and edgeR(3.32.1) https://cran.r-project.org/web/packages/pheatmap). (G–J) Consistency of the qRT‒PCR and RNA-seq results (n = 3 samples per group). (K–N) Consistency of the Western blot and RNA-seq results (n = 6 samples per group). (O, P) Quantitative analysis of the ACSL4 protein levels in STZ-induced DCM mice with reduced MARK4 expression (n = 4 samples per group). The data are presented as the means ± SDs. Significant differences are indicated by *P < 0.05, **P < 0.01, and ***P < 0.001.

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