Figure 4 | Scientific Reports

Figure 4

From: Pressurized liquid extraction followed by high-performance liquid chromatography for determination of beta-ecdysone extracted from Pfaffia glomerata (Spreng.) Pedersen

Figure 4

FT-Raman spectra from PLE extracts of leaf, stem, root and 20E samples. Extraction conditions: (A) leaf: T1—flow rate 2.0 mL min−1 and 80 °C; T2: flow rate 1.5 mL min−1 and 80 °C; T4—flow rate 2.0 mL min−1 and 60 °C; T5—flow rate 1.5 mL min−1 and 60 °C. (B) Stem: T10—flow rate 1.5 mL min−1 and 80 °C; T11—flow rate 2.0 mL min−1 and 80 °C; T12—flow rate 2.0 mL min−1 and 60 °C; T13—flow rate 1.5 mL min−1 and 60 °C. (C) Root: T24—flow rate 1.5 mL min−1 and 80 °C; T25—flow rate 2.0 mL min−1 and 80 °C; T26: flow rate 1.5 mL min−1 and 60 °C; T27—flow rate 2.0 mL min−1 and 60 °C. (D) β-ecdysone (20E) standard main peaks.

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