Figure 6
MBP-sc5G2-6xHis produced from HEK293FT cells. (a) cDNA sequence of MBP-sc5G2-6xHis. The underlined DNA sequence encodes the (G4S)3 linker sequence. (b) MBP-sc5G2-6xHis produced by HEK293FT was subjected to SDS-PAGE. The proteins were stained with CBB (left) and analyzed by western blot using anti-MBP antibody and anti-mIgG-HRP (right). M: Molecular weight marker. (c) MBP-sc5G2-6xHis produced by HEK293FT (0.8 μg/mL) was confirmed to bind specifically to the S1 + S2 and the S2 subunit of the recombinant spike protein by western blot. The bound MBP-sc5G2-6xHis was detected by the anti-MBP antibody and anti-mIgG-HRP. M: Molecular weight marker. (d) MBP-sc5G2-6xHis produced by HEK293FT detects the S1 + S2 spike proteins in the Vero cells infected with SARS-CoV-2 WT and its variants, α, β, γ, BQ1.1, and BA.5 but not in the uninfected Vero cells (-) as confirmed by western blot using MBP-sc5G2-6xHis (0.8 μg/mL), anti-MBP antibody and anti-mIgG-HRP. (e) ELISA for MBP-sc5G2-6xHis reactivity to 1 × 105 of SASR-CoV-2 WT and its variants (α, β, γ, and ο of BQ1.1, and BA.5). PBS: no virus coating. The data represent mean ± standard error (SE). The statistical significance was determined by one-way analysis of variance (one-way ANOVA). **P < 0.01, ***P < 0.001.
