Figure 5

Immunofluorescence staining of cell proliferation markers in the lung tissue of rats treated with MCT or PBS (Cont). (A) Immunofluorescence staining of PCNA and α-SMA. Scale bar = 50 μm. (B) TSA (tyramide signal amplification)-based immunofluorescence staining of Cyclin A2, α-SMA and CD31. iFluor® 488 tyramide was used for Cyclin A2, Cy5 tyramide was used for α-SMA and CD31. Scale bar = 20 μm.