Figure 3
From: Primary oocytes with cellular senescence features are involved in ovarian aging in mice
Effect of ABT263 treatment on oocyte maturation and fertility. (A) Oocytes from 9-month-old control mice (control 9 m) and ABT263-treated mice (ABT263 3-9 m) were stained with an antibody to γ-H2AX to detect DNA damage. DNA was revealed by DAPI staining. (B) Numbers of γ-H2AX-positive foci in the oocytes isolated from control ovaries and ABT263-treated ovaries at 9 months. (C) Brightfield microscopic images showing that oocytes isolated from control ovaries and ABT263-treated ovaries at 9 months underwent germinal vesicle breakdown (GVBD), and more oocytes isolated from ABT263-treated ovaries completed first polar body extrusion (PBE, arrows). (D, E) Percentages of oocytes underwent GVBD (D) and PBE (E). (F) Examples of meiotic spindles observed in oocytes isolated from control ovaries and ABT263-treated ovaries at 9 months. (G–I) The width of the metaphase I plate (MW) (G), the width of the meiotic spindle (SW) (H), and the length of the meiotic spindle (SL) (I) in the oocytes isolated from control ovaries and ABT263-treated ovaries at 9 months. (J–K) Number of deliveries (J) and number of pups (K) of each control mouse and ABT263-treated mouse during fertility assay. Data in the graph are presented as mean ± SD. *Represents significant difference; ns represents no significant difference. Three experimental replicates (n = 5 mice in each replicate) were conducted for analyses in graphs (B–I). n = 10 mice from the control and ABT-treated groups were used for fertility assay in graphs (J) and (K).
