Figure 2

STING facilitated plasmacytoid dendritic cells and macrophage expansion in pristane-induced lupus. The isolated splenocytes from WT and Sting^Gt mice were stained and analyzed by flow cytometry. Data show the proportion and the cell number of (A,B) DC (CD11c⁺), (C,D) CD11b⁺ Ly6g⁺ cells (neutrophils) out of splenocytes. The gating strategy of (E) CD11b⁺ CD11c⁺ mPDCA^- IAb⁺ (conventional dendritic cells or cDCs) population and (H) CD11c⁺ CD11b^- mPDCA⁺ (plasmacytoid dendritic cells or pDCs). The proportion and cell number of the (F,G) cDCs out of CD11c⁺CD11b⁺), (I,J) pDCs out of CD11c⁺CD11b^-, and (K,L) CD11c^- F4/80⁺ (macrophages) out of splenocytes. (M,N) The mean fluorescence intensity (MFI) of the IAb expression in the (M) CD11c⁺ cell and (N) CD11c^- F4/80⁺ population. Cell numbers are shown per spleen. Data are shown as mean ± SD; p-values were indicated *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Dot ● indicates control, and square ■ indicates PIL (N = 16–18 mice/group).