Figure 3
The effects of exogenously transfected SRSF3-FL and SRSF3-PTC mRNAs on endogenous SRSF3-FL and SRSF3-TR proteins were examined in HeLa cells. HeLa cells were transiently transfected with 0.5 µg of the pSG5.HA vector, pSG5.HA.SRSF3–FL, or pSG5.HA.SRSF3–PTC. After 16 h, the transfected cells were treated with 0.25 µM digoxin, 10 mM caffeine, or both in combination for 24 h. (A) These cells were collected and subjected to immunoblot analysis for the detection of the indicated proteins and ACTN (a loading control). Exo: exogenous; Endo: endogenous. (B) These cells were collected and subjected to RT‒PCR for the detection of the indicated mRNAs and GAPDH (a loading control).
