Figure 4
The effects of digoxin on the protein and mRNA stability of SRSF3-FL and SRSF3-TR in HeLa cells. HeLa cells were treated with 0.25 µM digoxin, 10 mM caffeine, 100 nM thapsigargin, or combinations of two or all three components for 24 h. (A) These cells were collected and subjected to immunoblot analysis for the detection of the indicated proteins and ACTN (a loading control). (B) mRNA was extracted and analyzed via RT‒PCR (with GAPDH as a loading control).
