Figure 5
Effects of LAP*, LAP, and LIP on the expression of SRSF3 in HeLa cells. (A) HeLa cells were transfected with 0.25 μg of SRSF3–LUC and 0.25 μg of the indicated genes. Luciferase activities in the transfected cell extracts were then determined (RLU indicates relative light units). These data are the average of three independent experiments (mean ± S.D.; n = 3). Student’s t tests were performed, and the results were compared with those obtained with the HA vector alone. * p < 0.05, ** p < 0.01, and *** p < 0.001. HeLa cells were transiently transfected with 0.5 μg of pSG5.HA.LAP, LAP*, and LIP. HeLa cells were treated with 10 mM caffeine or 0.25 μM digoxin for 24 h. (B) mRNA was extracted and analyzed via RT‒PCR (with GAPDH as a loading control). (C) These cells were collected and subjected to immunoblot analysis for the indicated proteins and ACTN (a loading control). The results are representative of two independent experiments.
