Figure 7
The effects of phosphate ions on the protein and mRNA stability of SRSF3 and p53 in HeLa cells. HeLa cells were treated with the indicated concentrations of phosphate ions for 24 h. (A) These cells were collected and subjected to immunoblot analysis for the detection of the indicated proteins and ACTN (a loading control). (B) mRNA was extracted and analyzed via RT‒PCR (with GAPDH as a loading control). (C) HeLa cells were treated with 4 mM phosphate ions plus 1 μM ActD for the indicated time for a total of 24 h. The mRNA was extracted and analyzed via RT‒PCR (with GAPDH as a loading control). (D) HeLa cells were treated with 0.5 mM phosphate ions plus 10 μg/ml CHX for the indicated times. (E) HeLa cells were pretreated with 5 mM NAC for 2 h and then treated with the indicated amount of phosphate ions for 24 h. These cells (D and E) were collected and subjected to immunoblot analysis for the detection of the indicated proteins and ACTN (a loading control).
