Figure 1
From: Glucose transporter-2 regulation of VMN GABA neuron metabolic sensor and transmitter gene expression
Effects of Ventromedial Hypothalamic Nucleus (VMN) Glucose Transporter-2 (GLUT) Gene Silencing on Dorsomedial VMN (VMNdm) or Ventrolateral VMN (VMNvl) γ-Aminobutyric Acid (GABA) Neuron Glutamate Decarboxylase-1 (GAD1/GAD67) and GAD2/GAD Gene Expression in the Female Rat. Groups of adult ovariectomized, estradiol-implanted female rats (n = 6/group) were pretreated by bilateral stereotactic administration of control/scramble (SCR) or GLUT2 siRNA to the VMN, then injected subcutaneously (sc) with vehicle (V) or neutral protamine Hagedorn insulin (INS; 10.0 U/kg bw) one hour before sacrifice. Individual VMNdm and VMNvl GABAergic neurons were labeled in situ by immunocytochemical methods for GAD65/67-immunoreactivity (-ir) prior to laser-catapult-microdissection for single-cell multiplex qPCR analysis. mRNA data were normalized to the housekeeping gene GAPDH by the 2-ΔΔCt method. Data are presented here in box-and-whisker plot format, which displays the median, lower and upper quartiles, and lower and upper extremes of a data set. Plots depict normalized GAD1/GAD67 [A (VMNdm) and C (VMNvl)] or GAD2/GAD65 mRNA [B (VMNdm) and D (VMNvl) values for GABAergic neurons collected from the following treatment groups: SCR siRNA/sc V (blue box-and-whisker plots, n = 12), GLUT2 siRNA/sc V (yellow box-and-whisker plots; n = 12), SCR siRNA/ sc INS (green box-and-whisker plots; n = 12), GLUT2 siRNA/sc INS (orange box-and-whisker plots; n = 12). Statistical differences between discrete pairs of treatment groups are denoted as follows: *p < 0.05; **p < 0.01; ***p < 0.001. (A) VMNdm GAD1/GAD67. (B) VMNdm GAD2/GAD65. (C) VMNvl GAD1/GAD67. (D) VMNvl GAD2/GAD65.
