Figure 3
From: Effects of an OX2R agonist on migration and removal of tau from mouse brain
Effects of OX-201 on tau, lactate, and glucose levels in hippocampal ISF of C57BL/6J mice and human P301S tau Tg mice. (a) Time schedule for drug administration and ISF sampling in C57BL/6J mice and human P301S tau Tg mice. After baseline ISF collection, vehicle or OX-201 were orally administered at ZT4.5. The effects of OX-201 on (b) tau, (c) lactate, and (d) glucose levels in ISF of C57BL/6J mice. Vehicle-treated mice: n = 5; OX-201-treated mice: n = 8. (e) Basal levels of ISF tau were collected for nonTg and human P301S tau Tg mice. NonTg mice: n = 10; Tg mice: n = 21. The effects of OX-201 on (f) tau, (g) lactate, and (h) glucose levels in ISF of human P301S tau Tg mice. Data were shown as a percentage of the basal tau level (average ISF tau levels at ZT0–4.5) in each mouse and shown as a mean ± SEM in each group. Vehicle-treated nonTg mice: n = 10; vehicle-treated Tg mice: n = 9; OX-201-treated Tg mice: n = 12. As the mean residence time of OX-201 was 4.87 h in C57BL/6J mice (Supplementary Figure S1), statistical analysis was performed with the data for 4.5 h after administration of OX-201 using repeated measures ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. ISF interstitial fluid; SEM standard error of the mean; Tg transgenic; ZT zeitgeber time.
