Figure 3
From: Mutations found in cancer patients compromise DNA binding of the winged helix protein STK19
A basic patch centered on the helix WH3-H1 contributes to dsDNA binding. (a, b) Electrostatic surface analysis of the STK19 structure reveals a large patch of basic residues centered around the WH3-H1 helix. The involved residues are labeled. (c) Surface representation showing the distribution of conserved residues from ConSurf analysis23 with default settings (conservation among 150 homologous STK19 sequences). The relatively conserved basic residues are labeled. (a) and (c) are of the same view. (d) K186E and R200E/K201E/K203E mutations disrupt the STK19 DNA binding ability. Representative images of EMSA are shown.
