Figure 1

The images of SCS surgery. SCS electrode used in this study and experimental protocol (Experiment 1 and 2). (A) Experimental protocol of this study. Experiment 1 for control group, 2 Hz group, 50 Hz group, 300 Hz group (n = 7, respectively). In the stimulation group, the stimulation was initiated immediately after the surgery. Kainic acid was administered on day 2 and video monitoring was performed; the stimulation was continued for 1 week in the stimulation group after 6 h of recording, and video monitoring was done again on day 9 followed by sacrifice. Another group was also created in which kainic acid was administered on day 2 followed by EEG (n = 3). Experiment 2 for control group, 2 Hz group, 50 Hz group, 300 Hz group (n = 5, respectively). The rats were injected with kainic acid on day 2 to induce convulsions. They were sacrificed after 4 h for qRT-PCR. (B) An image showing the dorsal skin incision exposing the occipital bone and cervical spinal cord. (C–F) Images of a cathode and anode were implanted on the right occipital bone and C2 dura mater, respectively. (G, H) Images of the electrodes and stimulator used in this experiment. The stimulator is connected to the electrodes as shown in the figure. (I) The electrodes were led over the back skin and connected to the stimulator before being protected by a handmade jacket.