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Figure 1

From: Identification of a urinary CD276 fragment for detecting resectable pancreatic cancer using a C-terminal proteomics strategy

Figure 1

Measurement of C-terminal tryptic fragments. (a) Proteomic workflow for quantitative analysis of protein C-termini. (b) Trypsin digestion in the presence of 18O-water. Trypsin digestion results in 18O incorporation into internal fragments, but not into C-terminal fragments. (c) Distinction of C-terminal fragments from internal fragments by MALDI-TOF MS. Internal fragments can be recognized by their unusual isotopic distribution due to 18O. (d) Enrichment of C-terminal fragments by SCX chromatography. In the purified fraction, peptides from protein C-termini were predominant, as judged by isotope distribution. MALDI-TOF MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, SCX strong cation exchange.

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