Figure 6

Annexin-V/PI double-staining in mouse MII oocytes in control, Ch-IONPs, and G-IONPs. (A) A bright field, a red signal in the nucleus (PI positive), and a green signal in the cytoplasmic membrane (Annexin-V positive). Scale bar 100 μm. (B) Quantification of viability analysis. Asterisks mean a significant difference (*p < 0.05). The ns indicates that it is not significant. G-IONPs green synthetized iron oxide nanoparticles, Ch-IONPs chemically synthetized iron oxide nanoparticles.