Figure 8 | Scientific Reports

Figure 8

From: Oocyte maturation, fertilization, and embryo development in vitro by green and chemical iron oxide nanoparticles: a comparative study

Figure 8

Intracellular ROS levels were evaluated with DCFH-DA and DHE in MII oocytes. (A) The mean fluorescence intensity of oocytes. (B) Representative photomicrographs of DCFH-DA staining. (C) The mean fluorescence intensity of oocytes. (D) Representative photomicrographs of DHE staining. (E) Extracellular ROS levels were assayed with DCFH-DA in the MII culture medium. The mean fluorescence intensity of the culture medium. Means ± SEM present the mean fluorescence intensity of the culture medium. Asterisks mean a significant difference (*p < 0.05, ****p < 0.0001). DCFH-DA 2′, 7′-dichlorofluorescein diacetate, DHE dihydroethidium.

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