Figure 2 | Scientific Reports

Figure 2

From: Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation

Figure 2

Relationship between neutron intensity and the appearance of 5-FOA resistance strain. Panel (a) relationship between high-energy neutrons and the appearance of 5-FOA resistance strain. Algal cultures were irradiated with high-energy neutron beams at indicated absorbed doses using the facility shown in Fig. 1a. The cells were then plated to the medium containing 5-FOA, and the number of resistant strain colonies was counted after a few weeks of incubation. Relative values are shown when the number with the highest appearance number is set to 1.0. n = 3, t-tests comparing the highest value (20 Gy) and the other ones; **p < 0.01; *p < 0.05. Panel (b) relationship between thermal neutrons and the appearance of 5-FOA resistant strains. The others are the same as panel (a), but algal cultures were irradiated with thermal neutrons by the facility shown in Fig. 1b.

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