Table 1 Comparison of breeding techniques and media additives.
Technique | Method | Advantage | Disadvantage |
|---|---|---|---|
Random mutagenesis | Chemical mutagen | Inclusion of unknown metabolic pathways as modification targets No restriction on outdoor culture Stable maintenance of strains | Need for establishing effective mutation introduction methods Need for establishing mutant screening methods Accompanying irrelevant mutations |
UV | |||
Gamma ray/X-ray | |||
Heavy ion beam | |||
Neutron (this study) | |||
Genome editing | ZFN | Planned phenotype improvement Stable maintenance of strains | Need for a deep understanding of metabolic pathways Need for genome sequencing Need for establishing genome editing methods (Legal restrictions on outdoor culture) |
TALEN | |||
CRISPR-Cas9 | |||
Genetic engineering | Gene overexpression | Planned phenotype improvement | Need for a deep understanding of metabolic pathways Need for establishing genetic modification methods Instability of strains Legal restrictions on outdoor culture |
Media additive | Activator chemicals | Inclusion of unknown metabolic pathways as drug targets No hassle of maintaining strains | Need for establishing chemical screening methods Need for access to chemical libraries Need for running costs in the production |
Inhibitor chemicals |
