Table 2 Temperature of real time-PCR reactions.

From: Trigonelline as an anticonvulsant agent: mechanistic insights into NMDA receptor expression and oxidative stress balance

Gene name

Hold (°C-min)

Cycling

Melt (°C–°C)

(°C-s)

Number of cycles

B2m

95–10

95–10

56–15

72–20

40

72–95

NR2A

95–10

95–10

62–15

72–20

40

72–95

NR2B

95–10

95–10

62–15

72–20

40

72–95

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