Figure 3 | Scientific Reports

Figure 3

From: Production of norovirus-, rotavirus-, and enterovirus-like particles in insect cells is simplified by plasmid-based expression

Figure 3

Accumulation compartments of VP6 produced in insect cells with BEVS or plasmid-base system. (A) SDS-PAGE and Western blot analyses of VP6 produced with BEVS. The left panel shows the stain-free total protein staining of the intracellular and extracellular samples from BEVS-based production. The right panel shows VP6 detection by Western blot using a commercial mouse VP6 IgG2a kappa monoclonal antibody. 15 µl of each sample was loaded per well as follows (1) intracellular BEVS-based sample and (2) extracellular BEVS-based sample. (B) SDS-PAGE and Western blot analyses of VP6 produced with plasmid-based system. The left panel shows the stain-free total protein staining of the intracellular and extracellular samples from plasmid-based production. The right panel shows VP6 detection by Western blot using a commercial mouse VP6 IgG2a kappa monoclonal antibody. 15 µl of each sample was loaded per well as follows: (1) intracellular plasmid-based sample and (2) extracellular plasmid-based sample. The gels and blots were cropped for clarity. For full photos, see Supplementary Fig. 3.

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