Figure 4 | Scientific Reports

Figure 4

From: Production of norovirus-, rotavirus-, and enterovirus-like particles in insect cells is simplified by plasmid-based expression

Figure 4

Characterisation of VP6 nanostructures after purification. A) SDS-PAGE and Western blot analyses of purified VP6s. The left panel shows the stain-free total protein staining of the purified VP6s. The right panel shows VP6 detection by Western blot using a commercial mouse VP6 IgG2a kappa monoclonal antibody. 15 µl of each purified VP6 was loaded per well as follows 1) Intracellular plasmid-based VP6 (c = 0.262 mg/ml, loaded 3.93µg) , 2) BEVS-based intracellular VP6 (c = 0.094 mg/ml, loaded 1.41µg) and 3) BEVS-based extracellular VP6 (c = 0.277 mg/ml, loaded 4.16µg). B) Dynamic light scattering analysis of the purified VP6s. C) Transmission electron microscopy (TEM) image of the sucrose gradient ultracentrifugation purified VP6 produced with plasmid-based system. Scale bar 200 nm, 50,000 × magnification. D) Representative TEM image of the sucrose gradient ultracentrifugation purified BEVS-based VP6. Scale bar 200 nm, 30,000 × magnification. The gels and blots were cropped for clarity. For full photos, see Supplementary Fig. 4.

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