Table 1 Yields of more than 95% pure noro-VLP by different expression methods. In BEVS, the amount of noro-VLP in the intracellular compartment is negligible, and we have established earlier that it is most efficient to collect noro-VLP from the production medium there.

From: Production of norovirus-, rotavirus-, and enterovirus-like particles in insect cells is simplified by plasmid-based expression

Noro-VLP expression method

Culture length

Source

Yield (mg/l)

Transfected

5 days

Intracellular

20 ± 0.2

Transfected

5 days

Extracellular

1.2

Transfected

9 days

Extracellular

42

BEVS

5 days

Extracellular

19 ± 13