Table 1 Yields of more than 95% pure noro-VLP by different expression methods. In BEVS, the amount of noro-VLP in the intracellular compartment is negligible, and we have established earlier that it is most efficient to collect noro-VLP from the production medium there.
Noro-VLP expression method | Culture length | Source | Yield (mg/l) |
|---|---|---|---|
Transfected | 5 days | Intracellular | 20 ± 0.2 |
Transfected | 5 days | Extracellular | 1.2 |
Transfected | 9 days | Extracellular | 42 |
BEVS | 5 days | Extracellular | 19 ± 13 |