Table 2 Pure CVB3-VLP yields after 30% sucrose cushion ultracentrifugation of the extracellular protein fraction (from cleared supernatant).

From: Production of norovirus-, rotavirus-, and enterovirus-like particles in insect cells is simplified by plasmid-based expression

CVB3-VLP production details

Culture length

Yield from 40 ml (mg)

2% 3CD/98% P1

5 days

1.6

8 days

1.9

5% 3CD/95% P1

5 days

1.8

8 days

2.1

10% 3CD/90% P1

5 days

1.3

8 days

1.3

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