Figure 5
Clonal diversity controls results in leaner CLD process (a) Experimental outline of clonal diversity control during the cell line development (CLD) process. Cellular barcodes were used as proxy for cellular diversity and classified in two groups. The depletion arm contained 48 clones with 15 different barcodes, the enrichment arm 48 clones with 48 distinct barcodes. (b) Absolute values for antibody titer after protein A purification(left panel), glutamate levels (middle panel), and viable cell density (right panel) grouped by classification into depletion or enrichment arm of cellular diversity. (c) Average values for maximum titer simulation of three independent CLD processes when screening different amount of clones. The error bars represent the standard deviations of the simulated titer values.
