Figure 1 | Scientific Reports

Figure 1

From: CD248-expressing cancer-associated fibroblasts induce epithelial–mesenchymal transition of non-small cell lung cancer via inducing M2-polarized macrophages

Figure 1

CAFs from NSCLC strongly express CD248. (A) Typical representation of dual immunofluorescence (IF) staining demonstrating CD248 and α-SMA colocalization in human NSCLC and normal adjoining tissues (NATs). Scale bar, 50 μm, 20 μm. (B) CD248, α-SMA, PDGFRβ, Vimentin and FAP in extracted CAFs and NFs, as determined by western blotting. (C) qPCR analysis demonstrating the CD248, FAP and Vimentin contents in CAFs and NFs. We conducted three separate experiments, and the results were analyzed. Data provided as mean ± SD. (D) IF staining demonstrating CD248 and α-SMA expression in CAFs and NFs. Scale bar, 50 μm. To conserve both antibodies and membrane materials, the blots were cut prior to hybridization with antibodies. Original images of blots are presented in Supplementary Fig. 1.

Back to article page