Figure 2 | Scientific Reports

Figure 2

From: CD248-expressing cancer-associated fibroblasts induce epithelial–mesenchymal transition of non-small cell lung cancer via inducing M2-polarized macrophages

Figure 2

CD248-expressing CAFs induced macrophages polarized to M2-type macrophages. (A) Dual immunofluorescence (IF) staining demonstrating CD248, CD68 and CD206 colocalization in human NSCLC and normal adjoining tissues (NATs). Scale bar, 50 μm. (B) qPCR analysis showing the levels of IL-6, CD206 and TGF-β in M0 macrophages after they were cultured with the CM of CD248 knockdown or control CAFs. Three independent experiments were performed and analysed. Data are provided as the means ± SEMs. p values were determined by a two-tailed t-test. (C) ELISA showing the levels of THP-1 secreting TGF-β in the CM of CD248 knockdown or control CAFs treated. Five independent experiments were performed and analysed. Data are provided as the means ± SEMs. p values were determined by a two-tailed t-test. p < 0.0001.

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