Figure 4 | Scientific Reports

Figure 4

From: CD248-expressing cancer-associated fibroblasts induce epithelial–mesenchymal transition of non-small cell lung cancer via inducing M2-polarized macrophages

Figure 4

CD248-expressing CAFs induced M2-polarized macrophages to induce NSCLC cells EMT. (A) Western blot analysis illustrating the expression of E-cadherin, N-cadherin, Vimentin, Smad2/3, p-Smad2/3 and TWIST in A549 treated with conditional media of CAFs-sh-CON, CAFs-sh-CD248, CAFs-CD248OE treated THP-1 CM. Original blots are presented in Supplementary Fig. 2. (B) Western blot analysis illustrating the expression of E-cadherin, N-cadherin, Vimentin, Smad2/3, p-Smad2/3 and TWIST in NCI-H460 treated with conditional media of CAFs-sh-CON, CAFs-sh-CD248, CAFs-CD248OE treated THP-1 CM. To conserve both antibodies and membrane materials, the blots were cut prior to hybridization with antibodies. Original images of blots are presented in Supplementary Figs. 2, 3.

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