Figure 1

Illustration of primers’ positions for rs17713054 in the ENSG00000288720 gene. Two reactions were conducted in two tubes for each sample. In each reaction, Fc and Rc primers produced a 586 bp amplicon as an internal control, and the allele-specific primers (Rw or Rm) produced a 301 bp amplicon. Fc, Forward control primer; Rc, Reverse control primer; Rm, Reverse mutant primer; Rw, Reverse wild type primer.