Figure 2
Gel electrophoresis of the amplified products showed three different genotypes for rs17713054 in three patients. To detect A and G alleles two reactions were carried out in parallele for each DNA sample. All amplified products contain a 586 bp PCR product as an internal control to check the validity of the PCR rection, while amplification of the allele-specific products with a length of 301 bp is dependent on the genotype of the each patient. Every two wells show the genotyping of a DNA sample: 1. 100 bp Ladder, 2&3. Homozygote patient with GG genotype (lanes 2 and 3 are indicative of homozygosity for the wild-type (G) allele as no product was observed for the mutant (A) allele in lane 3), 4&5. Homozygote patient with AA genotype (lanes 4 and 5 are indicative of homozygosity for the mutant (A) allele as no product was observed for the wild-type(G) allele in lane 5), 6&7. Heterozygote patients with GA genotype (lanes 6 and 7 are indicative of heterozygosity for A and G alleles as both were amplified. 8. Blank (no DNA template).
