Table 4 Statistical analysis of BB design showing coefficients, t –and p-values for significant variables affecting on agarase production by Bacillus subtilis PI .
Fit statistics | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Std. Dev | 5.95 | R2 | 0.943 | ||||||
Mean | 90.77 | Adjusted R2 | 0.8147 | ||||||
C.V. % | 6.55 | Adeq Precision | 9.0701 | ||||||
ANOVA for quadratic model | Coefficients in terms of coded factors | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Source | Sum of Squares | df | Mean Square | F- value | p value | Coefficient estimate | Standard Error | 95% CI high | |
Model | 259.9 | 9 | 2339 | 7.350 | 0.0352 | 94.06 | 4.20 | 105.7 | significant |
A-Agar | 109.0 | 1 | 109.0 | 3.080 | 0.1540 | 3.690 | 2.10 | 9.53 | |
B-Peptone | 863.0 | 1 | 863.0 | 24.41 | 0.0078 | 10.39 | 2.10 | 16.2 | |
C-Yeast extract | 1231 | 1 | 1231 | 34.82 | 0.0041 | 12.40 | 2.10 | 18.2 | |
AB | 7.770 | 1 | 7.770 | 0.2199 | 0.6635 | − 1.390 | 2.97 | 6.86 | |
AC | 1.980 | 1 | 1.980 | 0.0560 | 0.8246 | − 0.7034 | 2.97 | 7.55 | |
BC | 95.24 | 1 | 95.24 | 2.690 | 0.1761 | − 4.880 | 2.97 | 3.38 | |
A2 | 20.96 | 1 | 20.96 | 0.5927 | 0.4843 | − 2.560 | 3.32 | 6.67 | |
B2 | 3.030 | 1 | 3.030 | 0.0856 | 0.7844 | − 0.9727 | 3.32 | 8.26 | |
C2 | 15.97 | 1 | 15.97 | 0.4516 | 0.5384 | − 2.230 | 3.32 | 7.00 | |
Residual | 141.4 | 4 | 35.36 | ||||||
Lack of Fit | 138.5 | 3 | 46.17 | 0.1823 | 15.81 | ||||
Pure Error | 2.920 | 1 | 2.920 | ||||||
Cor Total | 2480.5 | 13 | |||||||
