Figure 8
The effect of RNH1 on LPS-induced inflammation and non-canonical inflammasome activation in THP-1 macrophages. THP-1 macrophages exposed to 50 ng/ml LPS in the presence or absence of 640 U/ml RNH1 compared to unstimulated cells were analyzed regarding (A) TNFα protein concentrations after stimulation for 4 h determined using ELISA; (B) relative CASP5 mRNA expression after stimulation for 4 h quantified using ELISA; and (C) IL-1β protein concentration after stimulation for 4 and 24 h analyzed using ELISA. Data are represented as column bar graphs of the mean ± SEM. One-way ANOVA followed by Tukey’s test was used for multiple comparisons; *p < 0.05; ***p < 0.001; ****p < 0.0001; LPS, lipopolysaccharide; RNH1, ribonuclease inhibitor 1; TNFα, tumor necrosis factor α; CASP5, caspase-5; IL-1β, interleukin-1 β; neg. ctrl., negative control.
