Figure 4

Two major populations of BAL cells are distinguished on the basis of CD163 and CD14 expression. BAL cells were stained for expression of a range of cell surface molecules and assessed by flow cytometry. Live, single cells were gated as shown in Supplementary Fig. 1 and gates were set as described in Supplementary Fig. 2. (a) CD163 vs CD14, (b) CD16 vs CD14, (c) CD16 vs CD163. One representative example of n = 4 biological replicates.