Figure 6
From: Enhanced chondrogenic potential in GelMA-based 3D cartilage model via Wnt3a surface immobilization
Cells migrate to the top of the gel and differentiate in the 3D regenerative model. (a) Representative immunofluorescence 3D image of Y201 cells after 7 days of culture, showing cell migration and differentiation towards the top of the gel in basic conditions (left), and in presence of TGF-β3 (center) and kartogenin (right) (nuclei = blue; SOX9 = green). Scale bars = 100 µm. (b) Quantification of cell migration, showing a significant increase in migratory distance in TGF-β3-treated samples compared to basic and kartogenin counterparts (data shown as median in interquartile range ± min/max and + symbol indicates mean value; ****p < 0.0001). (c) Quantification of SOX9 positive cells in the upper region of the gel shows increased differentiation in TGF-β3-treated samples compared to basic and kartogenin counterparts (*p < 0.05).
