Figure 2
SLC14A1 is significantly downregulated in PCa at both the mRNA and protein levels. (a) SLC14A1 mRNA expression in PCa tissues and normal adjacent tissues based on unpaired or (b) paired from TCGA database. (c) Analysis of SLC14A1 mRNA expression in adjacent tissues and PCa tissues from GSE6099 dataset (Tomlins et al.). (d,e) Analysis of mRNA expression of SLC14A1 in PCa tissues with different Gleason score from GSE21032 (Taylor et al.) dataset and TCGA database. (f,g) Analysis of SLC14A1 mRNA expression in PCa tissues with different N stage or T stage. (h) Relative mRNA expression of SLC14A1 in cell lines of PCa analyzed from CCLE online database (https://sites.broadinstitute.org/ccle/). (i) Protein expression levels of SLC14A1 in PCa cell lines detected by western blotting, β-actin was used as internal loading control (n = 3, mean ± SD). (j) Analysis of mRNA expression levels of SLC14A1 in PCa cell lines detected by Real-time qPCR, 18S was used as internal loading control (n = 3, mean ± SD). (k) Representative pictures of SLC14A1 protein expression in PCa tissues and adjacent tissues detected by IHC from THPA online database (https://www.proteinatlas.org/). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.
