Figure 4
From: Activation of hTREK-1 by polyunsaturated fatty acids involves direct interaction
Variability in TREK-1 activation by PUFAs depends on the initial current I0. (A) Scatter plot representations of IPUFA/I0 as a function of I0. (B) Linearization of the IPUFA/I0 vs I0 relationship following log-transformation for each PUFA. The simple linear regressions (line) are represented with the 90% confidence interval (IC; dotted line) used to discard one of the C20:4 n-3 and C20:4 n-6 data points (boxes). A point is discarded only if it is out by more than twice the 90% IC. (C) Relationship between Log10(IC22:6 n-3/I0) and Log10(I0) at 0 mV in different cell lines: HEK hTREK-1 stable cell line #1 (orange), HEK hTREK-1 stable cell line #2 (red) and HEK 293 T cells transiently transfected with TREK-1 channel (pIRES2 KCNK2 WT 6 ng/µL)(purple). (D) Lack of correlation between the Y-intercept of the linear regressions obtained before and the acyl chain length, (E) and the number of double bounds, for each PUFA at 10 µM.
