Figure 2 | Scientific Reports

Figure 2

From: First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues

Figure 2

PCV4 ORF2 phylogenetic analysis and comparison of nucleotide percent identities. The phylogenetic tree of the two PCV4 ORF2 sequences obtained in the present study and 73 reference sequences was constructed using the maximum-likelihood method in Geneious Prime software with the bootstrap method of 1,000 replications (A). The range of nucleotide percent identities is summarized for the two US PCV4 ORF2 sequences in comparison to Spanish, South Korean, Thai, Chinese, and Malaysian sequences (B). ORF2 nucleotide sequences were translated using standard genetic code and were aligned using Clustal Omega in Geneious Prime. Comparison of PCV4 US ORF2 amino acids proposed for subtype classification (residues 27, 28, 212) to PCV4a-1, PCV4-a2, and PCV4b subtypes (C).

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