Figure 3
PCV4 in situ hybridization by RNAscope (red staining) targeting transcribed ORF2 mRNA revealed viral replication in the germinal centers of lymph node (top) and in inflammatory cells of the lamina propria of the small intestine (bottom). In the lymph node, CD20 B lymphocytes have a regular distribution in the germinal centers, and numerous macrophages (Iba-1) are present. Additionally, CD3 T lymphocytes are diffusely distributed in the paracortex and scattered detection is observed in the germinal centers. In the lamina propria, the predominant cells observed within this inflammatory response include macrophages (Iba-1) and CD3 T lymphocytes. A few resident CD20 B lymphocytes, located within the lamina propria, were also observed. Both sections of lymph node and intestinal lamina propria displayed in this figure were from Case 1 (Table 1).
