Figure 3
Effect of clotrimazole (CTZ) on inducing apoptosis in MM.1S and NCI-H929 multiple myeloma cells. (a) Flow cytometric analysis of the apoptosis rate in CTZ-treated cells stained with Annexin V-FITC/7-ADD. (b) Rate of apoptosis in CTZ-treated cells. (c) Western blot analysis of the expression of Bax and Bcl-2 in CTZ-treated cells. (d) Western blot analysis of the expression of cleaved caspase-3 and cleaved PARP in CTZ-treated cells. (e,f) Bax/Bcl-2 ratios and semiquantitative analyses of the relative protein expression of cleaved caspase-3 and cleaved PARP in MM.1S and NCI-H929 cells. (g,h) Western blot analysis of the expression of Bax and Bcl-2 in CTZ-treated MM.1S and NCI-H929 cells. The data are expressed as the mean ± standard deviation of three independent sets of assays conducted in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus controls. ns not significant (p ≥ 0.05).
