Figure 5
Regulation of NF-κB signaling pathway by CTZ in MM.1S and NCI-H929 cells. (a,c) Western blot analysis was performed to evaluate the levels of NF-κB pathway signaling components, including p65, p-p65, IκBα, and p-IκBα in MM.1S and NCI-H929 cells treated with CTZ. (b,d,e,f) Semiquantitative analysis of Western blot results showing the expression levels of p65, p-p65, IkBa, and p-IκBα in MM.1S and NCI-H929 cells after CTZ treatment. (g,h) Quantitation of the p-IκBα/IκBα and p-p65/p65 ratios in MM.1S and NCI-H929 cells treated with different concentrations of CTZ. The data are presented as the mean ± standard deviation from three independent experiments conducted in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns not significant (p ≥ 0.05).
