Figure 4
LILRB4 knockdown promotes contractile phenotype switching and ECM stabilization in AD cell models. (A) The mRNA level of LILRB4 in the control, PDGF-BB, PDGF-BB + siNC, PDGF-BB + si-LILRB4-1, and PDGF-BB + si-LILRB4-2 groups was detected by RT-qPCR. The data are expressed as mean ± standard deviation (SD) from three biological replicates. One way ANOVA followed by Tukey’s post-hoc test: **P < 0.01 vs. Control group, ##P < 0.01 versus PDGF-BB + si-NC group. (B) Western blot analysis was conducted to assess the levels of contractile proteins, including α-SMA and SM22α, synthetic proteins S100A4, as well as ECM-related proteins LOX, MMP2, and MMP9 in the control, PDGF-BB, PDGF-BB + si-NC, and PDGF-BB + si-LILRB4 groups. Data are represented as mean ± standard deviation (SD) of three independent experiments. One way ANOVA followed by Tukey’s post-hoc test: **P < 0.01 versus Control group; Unpaired Student's t test: ##P < 0.01 versus PDGF-BB + si-NC group.
