Figure 8
Expression of the characterized genes in the intestinal mucosa of DSS-induced colitis mice and UC patients and the miRNA-mRNA-TF network. (a) Differences in the expression of feature genes in UC and healthy individuals were analyzed in the training set. (b) Validation of the expression differences in the characterized genes in UC and healthy individuals in the validation set. (c) The qRT-PCR to detect the mRNA expression levels of the characterized genes in the intestinal mucosa of normal control and DSS-induced colitis mice. (d) The qRT-PCR detection of the mRNA expression levels of the characterized genes in the intestinal mucosa of healthy individuals and UC patients. (e,f) Western blotting to detect the protein expression levels of the characterized genes in the intestinal mucosa of normal control and DSS-induced colitis mice. (g,h) Western blotting to detect the protein expression levels of the characterized genes in the intestinal mucosa of healthy people and UC patients. (The samples derive from the same experiment and that blots were processed in parallel. We cropped the membranes according to the molecular weight of the target proteins, resulting in the absence of some images of adequate length. Therefore, we uploaded the original images of all target proteins for human and animal samples in the supplementary material.) (i) The miRNA-mRNA-TF regulatory network diagram (red circles indicate the characterized genes, yellow diamonds indicate TFs, and blue triangles indicate miRNAs).
