Figure 1

Chemical structure of tHGA and experimental design. (a) The chemical structure of 2,4,6-trigydroxy-3-geranyl acetophenone (tHGA) contains a phloroglucinol structural-core as the bioactive principle. (b) Experimental design: Mice were sensitised with 1% DNCB on day 1. From day 8, mice were challenged with 0.5% DNCB three times per week up to 4 weeks. In the final 2 weeks, orally treated mice were administered different doses of tHGA, drug control or vehicle 1-h prior to DNCB application for 3 days per week, while topical treated mice were applied with different doses of tHGA, drug control or vehicle 4-h prior to DNCB application daily. Mice were observed for scratching behaviour on day 33 and evaluated for SCORAD on day 34 before being sacrificed for blood and skin sample collection.