Table 1 High-quality clean reads of iRIP-seq.

From: KPNA2 promotes the progression of gastric cancer by regulating the alternative splicing of related genes

Sample ID

RAW

Clean

Raw base

Clean base

Unique Tag

Q20 (%)

Q30 (%)

DUP (%)

KPNA2_IP_1

41648652

39899321 (95.80%)

6.25G

5.41G (86.62%)

9450813 (23.69%)

98.25

94.89

93.27

KPNA2_IP_2

40714564

39044923 (95.90%)

6.11G

5.30G (86.86%)

8970225 (22.97%)

98.18

94.72

93.61

KPNA2_input_1

70240752

69171773 (98.48%)

10.54G

7.78G (73.86%)

13739402 (19.86%)

98.64

95.54

95.98

KPNA2_input_2

54821866

53893644 (98.31%)

8.22G

5.86 (71.29%)

11426018 (21.20%)

98.63

95.52

95.64

  1. (1) RAW: Number of raw sequences transformed from primary image data obtained by base-call sequencing; (2) Clean: Stripping adapter sequences from raw reads, the number of valid sequences acquired after low quality bases for subsequent analysis; (3) Raw base: Based on the length and number of the raw reads, calculate the number of bases they contained, in G; (4) Clean base: Depending on the number and length of the clean reads, the number of bases they contain was calculated, in G; (5) Ratio of the number of non-repeat reads to their clean reads.; (6) Q20: The proportion of bases with sequencing error rates below 1%.; (7) Q30: The proportion of bases with sequencing error rates below 0.1%; (8) DUP: Duplication level. Ratio of duplicate reads to total reads.
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