Figure 7
Quorum sensing response of P. aeruginosa cells in alternating signal on/off periods, with 1 µM maximal signal molecule concentration. (a) The red line shows the population-level average of the fluorescence intensity (the shaded area represents the standard deviation). The dashed black line indicates the threshold intensity (23.1 a.u.). Model calculation of the average fluorescence intensity data is presented by a black line, where the model parameters from Table 1 were used. See Supplementary Fig. S13b for the corresponding cell numbers. (b) Coefficient of variation (continuous line) calculated based on the mean fluorescence intensity and its standard deviation, and the standard deviation (dotted line) is presented as a function of time. (c) Fraction of QS-on cells within the device during the experiment. (d) The average normalized fluorescence intensity difference for pairs of cells concurrently present in the device as a function of their cell lineage distance. See Supplementary Fig. S13c for the number of cell pairs analyzed. (e) Probability of being in opposite QS states for pairs of cells concurrently present in the device as a function of their cell lineage distance.
