Figure 2 | Scientific Reports

Figure 2

From: Taurine, alpha lipoic acid and vitamin B6 ameliorate the reduced developmental competence of immature mouse oocytes exposed to methylglyoxal

Figure 2

Supplementation of IVM medium with TAB cocktail (Taurine, ALA, and B6) improves the developmental competence of matured COCs exposed to MGO stress. COCs were cultured in IVM medium with indicated concentrations of MGO (75µM) and/or TAB (Taurine: 10 mM; ALA: 10 µM and B6: 10 µM) for 16–18 and then IVF was carried out on matured COCs and then cultured in G-TL medium for 4.5 days; (A) Maturation rate/ GV, (B) 2PN formation rate/ GV and (C) Blastocyst rate/ GV; At least 50 COCs were cultured in each replicate of each treatment group. Five replicates were included. Data are presented as mean ± SEM. Different letters (a and b) indicate significant differences between the treatment groups at P < 0.05. Statistical differences between groups were assessed using one-way ANOVA with LSD post-hoc test.

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